Rashtrasant Janardhan Swami
Smt. Sakhubai Narayanrao Katkade medical college & Research Centre
Kokamthan, Tal- Kopargaon, Dist. Ahmednagar, Maharashtra -423601.
• To deliver advanced knowledge across all areas of Clinical Microbiology through
impactful education, ethical research, and by fostering self-learning among students.
• To offer top-quality laboratory diagnostic services to enhance patient care.
• To promote awareness about the prevention of healthcare-associated infections and
emphasize the rational use of antibiotics..
• Provide in-depth knowledge about the causative agents of infectious diseases to
facilitate early diagnosis.
• Motivate students to develop self-learning skills, enabling them to become competent and independent learners.
• Enhance knowledge on the prevention and control of healthcare-associated infections
to minimize the burden on healthcare systems and improve patient outcomes by
reducing morbidity and mortality.
• Raise awareness about bacterial drug resistance and promote the rational use of
antibiotics to curb the emergence of resistant strains.
• Conduct fundamental and applied research aimed at benefiting the community.
1. Laboratory Size & Capacity
• Should accommodate 50 students per batch (for a 150-seat MBBS college, three batches are required).
• Minimum area: 1500–2000 sq. ft.
• Well-ventilated, well-lit, and with proper safety measures.
2. Essential Equipment & Infrastructure
Microscopy & Basic Equipment
• 50+ Binocular Microscopes (one per student).
• 1-2 Teaching Microscopes with projection capabilities.
• Glass Slides, Cover Slips, and Staining Racks for bacterial, fungal, and parasitic staining.
Culture & Incubation
• Bacteriological Incubator for microbial growth.
• Laminar Air Flow (Biosafety Cabinet) for aseptic work.
• Anaerobic Jar for anaerobic bacterial cultures.
• Refrigerator (4°C) & Deep Freezer (-20°C) for media and sample storage.
Sterilization & Disinfection
• Autoclave & Hot Air Oven for sterilization.
• Spirit Lamps & Bunsen Burners for flame sterilization.
• Disinfectant Solutions & Handwashing Stations for biosafety.
Media Preparation & Staining
• Culture Media (Nutrient Agar, MacConkey, Blood Agar, etc.)
• Gram Staining & Ziehl-Neelsen (ZN) Staining Kits
• Sterile Petri Dishes, Test Tubes, Pipettes, and Loops
3. Safety & Waste Disposal
• Proper Ventilation & Fume Hood to handle stains and chemicals.
• Biohazard Waste Disposal System (separate bins for biological and chemical waste).
• Fire Extinguishers & First Aid Kit for emergency situations.
4. Teaching & Learning Resources
• Digital Microscope with Screen Projection for demonstrations.
• Illustrated Charts, Models & E-Learning Resources.
• Specimen Bank (slides of bacteria, fungi, parasites).
5. Practical Exercises for MBBS Students
• Microscopy: Identification of bacteria, fungi, and parasites.
• Staining Techniques: Gram staining, Acid-fast staining (AFB), etc.
• Culture Techniques: Streak plate method, antibiotic sensitivity testing.
• Serological Tests: Widal, VDRL, ELISA, Rapid antigen tests.
• Hand Hygiene & Aseptic Techniques: Infection control practices.
S.No. | NAME OF THE ITEMS | No. Required |
---|---|---|
(A) General: | ||
1 | Anaerobic apparatus | 2 |
2 | Autoclave | 3 |
3 | Balance Electronic Digital | 2 |
4 | Biosafety Cabinet Type - 2A | 3 |
5 | BOD Incubator | 1 |
6 | Centrifuge | 4 |
7 | CO2 Incubator/Candle Jar | 2 |
8 | Computer Unit | 3 |
9 | Deep Freeze -20° C & Deep Freezer | 1 each |
10 | Distilled water Plant | 1 |
11 | Elisa Reader | 2 |
12 | Elisa Washer | 2 |
13 | Hot Air Oven | 2 |
14 | Incubator | 3 |
15 | Lab Refrigerator (minimum 400 litres) | 4 |
16 | Laminar flow | 1 |
17 | Micrometer eye pieces | 2 |
18 | Micrometer stage | 2 |
19 | Microscope Binocular (Students) | 90 |
20 | Microscope Binocular | Every Faculty |
21 | Microscope with universal condenser containing oil immersion, Bright field, Phase Contrast & Dark ground | 1 |
22 | Multimedia Projector | 2 |
23 | pH determination apparatus | 2 |
24 | Serum inspissators | 1 |
25 | VDRL shaker | 2 |
26 | Vortex Mixer | 2 |
27 | Water bath with variable temperature | 2 |
28 | Oil-immersion lens for student microscope | 50 |
29 | Automated Blood Culture System | 1 |
30 | Colony Counter | 1 |
(B) Consumables for Culture and Serological Diagnosis: | ||
31 | Antibiotic Discs for Antibiotic susceptibility testing | As required |
32 | Antibiotic zone scale | As required |
33 | Antisera-Salmonella | As required |
34 | Antisera-Shigelladysenteriae | As required |
35 | Antisera-Shigellaflexnari | As required |
36 | Antisera-Shiegellasonnie | As required |
37 | Antisera-Vibrio cholerae | As required |
38 | ATCC strain - Enterococcus fecalis 29213 | As required |
39 | ATCC strain - E.coli 25922 | As required |
40 | ATCC strain - E.coli 35218 | As required |
41 | ATCC strain - Pseudomonas aeruginosa 27853 | As required |
42 | ATCC strain - Staphylococcus aureus 25923 | As required |
43 | ATCC strain - Staphylococcus aureus 29213 | As required |
44 | Bottles for blood culture | As required |
45 | Micropipettes – Multi channel & Single channel | As required |
46 | Materials for preparation of various Culture Media | As required |
47 | Materials required for various Microbiological Stains | As required |
48 | Digital Thermometers of different temperatures | As required |
49 | Various Kits for Serological diagnostics | As required |
50 | Laptop | 1 |
51 | Desktop Computer, with Printer | 2 |
52 | Photocopier and Scanner | 1 |
Note: All equipment must be calibrated and maintained.
ISBN | Author | Title | Publisher |
---|---|---|---|
9789380316680 | Parija | Practical Microbiology for Undergraduate Medical Student (PB) | APH |
9788190401180 | Singh | Textbook of Microbiology | Globalmedik |
9789380316895 | Swamy | Comprehensive Medical Microbiology (CBME Curriculum) | APH |
9781032134529 | Fraise | Ayliffe's Control of Healthcare-Associated Infection, 5th Ed | CRC Press |
9781032391892 | Barh, Lundstrom | COVID-19 from Bench to Bedside (SIE) | CRC Press |
9789356963320 | Sastry | Essentials of Medical Microbiology, 4th Ed | JPB |
9781032290119 | Male | Immunology: An Illustrated Outline, 6th Ed (SIE) | CRC Press |
9780367206697 | Paul Elliott | Infection Prevention and Control: Perceptions and Perspectives | CRC Press |
9789394792180 | Baveja | Practical Microbiology for MBBS, 5th Ed | APC |
9789394102477 | Baveja | Textbook of Microbiology, 7th Ed | APC |
9789380316697 | Parija | Textbook of Practical Microbiology, 2nd Ed | APH |
9789388696661 | Kirmani | Washington Manual Infectious Diseases Subspecialty Consult, 3rd Ed (SAE) | LWW |
9780198810858 | Brent | Oxford Handbook of Tropical Medicine, 5th Ed | Oxford |
9780198846482 | Humphreys | Oxford Case Histories in Infectious Diseases and Microbiology, 3rd Ed | Oxford |
9780197784181 | Hardy | Fundamentals of HIV Medicine, 2023 Edition (SAE) | Oxford |
9789394102095 | Baveja | Complete Microbiology for MBBS, 1st Ed | APC |
9780198855576 | Damani | Manual of Infection Prevention and Control, 4th Ed (SAE) | Oxford |
9788131261194 | Nagoba | Medical Microbiology and Parasitology: Prep Manual for Undergraduates, 4th Ed | Elsevier |
9788123918105 | Chatterjee | Parasitology: Protozoology and Helminthology, 13th Ed | CBS |
9781319498658 | Stranford S. | Kuby Immunology COVID-19 and Digital Update, 8th Ed | Springer |
9780367371203 | Razzaghi-Abyaneh | Medical Mycology: Current Trends and Future Prospects | CRC Press |
9788119714537 | Vinod Kumar Swamy | Applied Microbiology and Infection Control, 1st Ed | APH |
9781555814700 | Sanders | I, Microbiologist: A Discovery-Based Undergraduate Research Course | ASM Press |
9789393330659 | Ananthanarayan | Ananthanarayan & Panikar's Textbook of Microbiology, 13th Ed | Orient Blackswan |
9781555813932 | Levy | HIV and Pathogenesis of AIDS, 3rd Ed | ASM |
9781555816735 | Lamont | Oral Microbiology and Immunology, 2nd Ed | ASM |
9781555813185 | Macrina | Scientific Integrity, 3rd Ed | ASM |
9789386827555 | ARORA D.R. | MEDICAL PARASITOLOGY 5ED (PB 2020) | CBS |
9781555814960 | COURVALIN | ANTIBIOGRAM{HB} | ASM |
9781555814762 | MITCHELL | CULTURAL HERITAGE MICROBIOLOGY : FUNDAMENTAL STUDIES IN CONSERVATION SCIENCE (HB) | ASM |
9781555813659 | THOMPSON | THE BIOLOGY OF VIBRIOS | ASM |
9780763756895 | KRASNER | THE MICROBIAL CHALLENGE, 2/E : SCIENCE, DISEASE AND PUBLIC HEALTH [PB] | ASM |
9781555814441 | SCHELD | EMERGING INFECTIONS- 8 | ASM |
9789814366823 | LEE, YUAN KUN | MICROBIAL BIOTECHNOLOGY: PRINCIPLES AND APPLICATIONS (THIRD EDITION) (REVISED) | WORLD SCIENTIFIC |
9781848162945 | LAYCOCK | PERSPECTIVES ON VASOPRESSIN | IMPERIAL COLLEGE PRESS |
9781555818784 | SPERAMDOP | ENTEROHEMORRHAGIC ESCHERICHIA COLI AND OTHER SHIGA TOXIN PRODUCING E COLI (HB 2015) | ASM |
9781555815028 | SABOUR | BACTERIOPHAGES IN THE CONTROL OF FOOD AND WATERBORNE PATHOGENS (HB) | ASM |
9780896033986 | U. REISCHL | MOLECULAR DIAGNOSIS OF INFECTIOUS DISEASES (METHODS IN MOLECULAR MEDICINE) | HUMANA |
9780896033672 | LIBERMAN | ALLERGIC DISEASES: DIAGNOSIS AND TREATMENT (PRIMARY CARE) | HUMANA |
9781555813741 | DOMINGO | MICROBIAL SOURCE TRACKING | ASM |
9781555813765 | STURCHLER | EXPOSURE: A GUIDE TO SORUCES OF INFECTIONS (PB) | ASM |
9789810241223 | TAN | STOCHASTIC MODELLING OF AIDS EPIDEMIOLOGY AND HIV PATHOGENESIS | WORLD SCIENTIFIC |
9780763707033 | DAIGLE | HIV HOMECARE HANDBOOK | JONES AND BARTLETT |
9781555812225 | FREDRICKSON | THE RECOMBINANT DNA CONTROVERSY:A MEMOIR | ASM |
9781555812645 | GEST | MICROBES : AN INVISIBLE UNIVERSE, REVISED EDN. {HB} | ASM |