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Rashtrasant Janardhan Swami
Smt. Sakhubai Narayanrao Katkade medical college & Research Centre Kokamthan, Tal- Kopargaon, Dist. Ahmednagar, Maharashtra -423601.

College Logo

Goals :


• To deliver advanced knowledge across all areas of Clinical Microbiology through impactful education, ethical research, and by fostering self-learning among students.
• To offer top-quality laboratory diagnostic services to enhance patient care.
• To promote awareness about the prevention of healthcare-associated infections and emphasize the rational use of antibiotics..




Objective :


• Provide in-depth knowledge about the causative agents of infectious diseases to facilitate early diagnosis.
• Motivate students to develop self-learning skills, enabling them to become competent and independent learners.
• Enhance knowledge on the prevention and control of healthcare-associated infections to minimize the burden on healthcare systems and improve patient outcomes by reducing morbidity and mortality.
• Raise awareness about bacterial drug resistance and promote the rational use of antibiotics to curb the emergence of resistant strains.
• Conduct fundamental and applied research aimed at benefiting the community.


Practical Hall & Laboratories:


1. Laboratory Size & Capacity
• Should accommodate 50 students per batch (for a 150-seat MBBS college, three batches are required).
• Minimum area: 1500–2000 sq. ft.
• Well-ventilated, well-lit, and with proper safety measures.

2. Essential Equipment & Infrastructure
Microscopy & Basic Equipment
• 50+ Binocular Microscopes (one per student).
• 1-2 Teaching Microscopes with projection capabilities.
• Glass Slides, Cover Slips, and Staining Racks for bacterial, fungal, and parasitic staining.

Culture & Incubation
• Bacteriological Incubator for microbial growth.
• Laminar Air Flow (Biosafety Cabinet) for aseptic work.
• Anaerobic Jar for anaerobic bacterial cultures.
• Refrigerator (4°C) & Deep Freezer (-20°C) for media and sample storage.

Sterilization & Disinfection
• Autoclave & Hot Air Oven for sterilization.
• Spirit Lamps & Bunsen Burners for flame sterilization.
• Disinfectant Solutions & Handwashing Stations for biosafety.

Media Preparation & Staining
• Culture Media (Nutrient Agar, MacConkey, Blood Agar, etc.)
• Gram Staining & Ziehl-Neelsen (ZN) Staining Kits
• Sterile Petri Dishes, Test Tubes, Pipettes, and Loops

3. Safety & Waste Disposal
• Proper Ventilation & Fume Hood to handle stains and chemicals.
• Biohazard Waste Disposal System (separate bins for biological and chemical waste).
• Fire Extinguishers & First Aid Kit for emergency situations.

4. Teaching & Learning Resources
• Digital Microscope with Screen Projection for demonstrations.
• Illustrated Charts, Models & E-Learning Resources.
• Specimen Bank (slides of bacteria, fungi, parasites).

5. Practical Exercises for MBBS Students
• Microscopy: Identification of bacteria, fungi, and parasites.
• Staining Techniques: Gram staining, Acid-fast staining (AFB), etc.
• Culture Techniques: Streak plate method, antibiotic sensitivity testing.
• Serological Tests: Widal, VDRL, ELISA, Rapid antigen tests.
• Hand Hygiene & Aseptic Techniques: Infection control practices.


Instrument & Equipment :


S.No. NAME OF THE ITEMS No. Required
(A) General:
1Anaerobic apparatus2
2Autoclave3
3Balance Electronic Digital2
4Biosafety Cabinet Type - 2A3
5BOD Incubator1
6Centrifuge4
7CO2 Incubator/Candle Jar2
8Computer Unit3
9Deep Freeze -20° C & Deep Freezer1 each
10Distilled water Plant1
11Elisa Reader2
12Elisa Washer2
13Hot Air Oven2
14Incubator3
15Lab Refrigerator (minimum 400 litres)4
16Laminar flow1
17Micrometer eye pieces2
18Micrometer stage2
19Microscope Binocular (Students)90
20Microscope BinocularEvery Faculty
21Microscope with universal condenser containing oil immersion, Bright field, Phase Contrast & Dark ground1
22Multimedia Projector2
23pH determination apparatus2
24Serum inspissators1
25VDRL shaker2
26Vortex Mixer2
27Water bath with variable temperature2
28Oil-immersion lens for student microscope50
29Automated Blood Culture System1
30Colony Counter1
(B) Consumables for Culture and Serological Diagnosis:
31Antibiotic Discs for Antibiotic susceptibility testingAs required
32Antibiotic zone scaleAs required
33Antisera-SalmonellaAs required
34Antisera-ShigelladysenteriaeAs required
35Antisera-ShigellaflexnariAs required
36Antisera-ShiegellasonnieAs required
37Antisera-Vibrio choleraeAs required
38ATCC strain - Enterococcus fecalis 29213As required
39ATCC strain - E.coli 25922As required
40ATCC strain - E.coli 35218As required
41ATCC strain - Pseudomonas aeruginosa 27853As required
42ATCC strain - Staphylococcus aureus 25923As required
43ATCC strain - Staphylococcus aureus 29213As required
44Bottles for blood cultureAs required
45Micropipettes – Multi channel & Single channelAs required
46Materials for preparation of various Culture MediaAs required
47Materials required for various Microbiological StainsAs required
48Digital Thermometers of different temperaturesAs required
49Various Kits for Serological diagnosticsAs required
50Laptop1
51Desktop Computer, with Printer2
52Photocopier and Scanner1

Note: All equipment must be calibrated and maintained.


Library :


ISBN Author Title Publisher
9789380316680ParijaPractical Microbiology for Undergraduate Medical Student (PB)APH
9788190401180SinghTextbook of MicrobiologyGlobalmedik
9789380316895SwamyComprehensive Medical Microbiology (CBME Curriculum)APH
9781032134529FraiseAyliffe's Control of Healthcare-Associated Infection, 5th EdCRC Press
9781032391892Barh, LundstromCOVID-19 from Bench to Bedside (SIE)CRC Press
9789356963320SastryEssentials of Medical Microbiology, 4th EdJPB
9781032290119MaleImmunology: An Illustrated Outline, 6th Ed (SIE)CRC Press
9780367206697Paul ElliottInfection Prevention and Control: Perceptions and PerspectivesCRC Press
9789394792180BavejaPractical Microbiology for MBBS, 5th EdAPC
9789394102477BavejaTextbook of Microbiology, 7th EdAPC
9789380316697ParijaTextbook of Practical Microbiology, 2nd EdAPH
9789388696661KirmaniWashington Manual Infectious Diseases Subspecialty Consult, 3rd Ed (SAE)LWW
9780198810858BrentOxford Handbook of Tropical Medicine, 5th EdOxford
9780198846482HumphreysOxford Case Histories in Infectious Diseases and Microbiology, 3rd EdOxford
9780197784181HardyFundamentals of HIV Medicine, 2023 Edition (SAE)Oxford
9789394102095BavejaComplete Microbiology for MBBS, 1st EdAPC
9780198855576DamaniManual of Infection Prevention and Control, 4th Ed (SAE)Oxford
9788131261194NagobaMedical Microbiology and Parasitology: Prep Manual for Undergraduates, 4th EdElsevier
9788123918105ChatterjeeParasitology: Protozoology and Helminthology, 13th EdCBS
9781319498658Stranford S.Kuby Immunology COVID-19 and Digital Update, 8th EdSpringer
9780367371203Razzaghi-AbyanehMedical Mycology: Current Trends and Future ProspectsCRC Press
9788119714537Vinod Kumar SwamyApplied Microbiology and Infection Control, 1st EdAPH
9781555814700SandersI, Microbiologist: A Discovery-Based Undergraduate Research CourseASM Press
9789393330659AnanthanarayanAnanthanarayan & Panikar's Textbook of Microbiology, 13th EdOrient Blackswan
9781555813932LevyHIV and Pathogenesis of AIDS, 3rd EdASM
9781555816735LamontOral Microbiology and Immunology, 2nd EdASM
9781555813185MacrinaScientific Integrity, 3rd EdASM
9789386827555ARORA D.R.MEDICAL PARASITOLOGY 5ED (PB 2020)CBS
9781555814960COURVALINANTIBIOGRAM{HB}ASM
9781555814762MITCHELLCULTURAL HERITAGE MICROBIOLOGY : FUNDAMENTAL STUDIES IN CONSERVATION SCIENCE (HB)ASM
9781555813659THOMPSONTHE BIOLOGY OF VIBRIOSASM
9780763756895KRASNERTHE MICROBIAL CHALLENGE, 2/E : SCIENCE, DISEASE AND PUBLIC HEALTH [PB]ASM
9781555814441SCHELDEMERGING INFECTIONS- 8ASM
9789814366823LEE, YUAN KUNMICROBIAL BIOTECHNOLOGY: PRINCIPLES AND APPLICATIONS (THIRD EDITION) (REVISED)WORLD SCIENTIFIC
9781848162945LAYCOCKPERSPECTIVES ON VASOPRESSINIMPERIAL COLLEGE PRESS
9781555818784SPERAMDOPENTEROHEMORRHAGIC ESCHERICHIA COLI AND OTHER SHIGA TOXIN PRODUCING E COLI (HB 2015)ASM
9781555815028SABOURBACTERIOPHAGES IN THE CONTROL OF FOOD AND WATERBORNE PATHOGENS (HB)ASM
9780896033986U. REISCHLMOLECULAR DIAGNOSIS OF INFECTIOUS DISEASES (METHODS IN MOLECULAR MEDICINE)HUMANA
9780896033672LIBERMANALLERGIC DISEASES: DIAGNOSIS AND TREATMENT (PRIMARY CARE)HUMANA
9781555813741DOMINGOMICROBIAL SOURCE TRACKINGASM
9781555813765STURCHLEREXPOSURE: A GUIDE TO SORUCES OF INFECTIONS (PB)ASM
9789810241223TANSTOCHASTIC MODELLING OF AIDS EPIDEMIOLOGY AND HIV PATHOGENESISWORLD SCIENTIFIC
9780763707033DAIGLEHIV HOMECARE HANDBOOKJONES AND BARTLETT
9781555812225FREDRICKSONTHE RECOMBINANT DNA CONTROVERSY:A MEMOIRASM
9781555812645GESTMICROBES : AN INVISIBLE UNIVERSE, REVISED EDN. {HB}ASM